Shot with D300 at f8 with AF-S 105mm Micro VR 2.8, SB-800 with diffuser right, SB-800 left with gobo to illumnate the white bristleboard background. There is an overhead halogen desk lamp providing ambient. This is a composite of 30 images, each with a slightly different focus. This was due to the fact that the flower is approximately 40mm in depth, and F8 has only slightly less that 2 mm of depth. The stacks were assembled in Zerene Stacker. TetherScript was used to capture the images for the focus stack.