The Legend of Zelda: Hyrule
Overworld of Hyrule from the video game The Legend of Zelda released for the Nintendo Entertainment System in 1986.
One stud represents 16x16 pixels. The map itself (without the bottom text) is 256 studs by 88 studs (each screen/section is 16x11).
First displayed at BrickCon 2012 in Art/Sculpture/Mosaic.
I made some changes over the weekend to make displaying / storage easier and fixed a couple errors.
Update 1: Awarded the "A Winner is You" trophy for the Let's Play theme at Bricks Cascade 2013.