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Microscope objectives in photography

Apart from a whole bunch of different microscope objectives, what you see above is a test image shot at 45:1 magnification and showing a small portion of a butterfly's wing. It was shot while trying out a new solution for high magnification stacking. Butterfly wings are well suited for tests like this since they provide fine detail across the entire frame without requiring very deep stacks.

 

60 exposures focus stacked in Zerene Stacker (PMAX).

Objective: Nikon CF Plan 50x 0.55 ∞/- EPI ELWD

Tube lens: $9 surplus lens

Camera: Canon 5DmkII

Lighting: One Nikon SB-24 diffused through a strip of white semitransparent plastic wrapping around the end of the objective.

 

Background: I'm writing this summary to have something to point to when people ask for advice regarding microscope objectives for photograpy. Consider it a living document – I have a feeling it will be subject to many changes and updates.

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Taken on January 22, 2011