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Figure2

Figure 2. Confocal images of ratite eggshells stained for DNA. (a) Confocal radial cross section of an Aepyornis eggshell, stained with SYBR Green, displaying the DNA distributed throughout the matrix: 5× objective lens, scale bar, 400 µm. Inset, orientation of confocal image. (b) Confocal inner surface of a D. robustus eggshell, stained with Hoechst dye, displaying mammillary cones (outlined) with peripherally located DNA: 40× objective lens, scale bar, 50 µm. Inset, orientation of confocal image. Red arrows, fluorescently labelled DNA.

DOI: 10.1098/rspb.2009.2019

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Uploaded on March 10, 2010