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母斑馬魚 Female Zebrafish-01_Worth US$200 dollars | by 阿鶴
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母斑馬魚 Female Zebrafish-01_Worth US$200 dollars

1101_2011_Update

 

Thanks for the German AtmoSAFE Company choosing this zebrafish photo named "Female Zebrafish-01_Worth $200 US dollars" on their official website homepage.

The homegape URL is " www.atmosafe.net/de.html ".

The application article is "Der Zebrafisch mag keinen Stress" and its URL is " www.atmosafe.net/de/anwendungen/bebrueten-und-zuechten/ze... "

 

0428.2011 Update

 

Thanks for the Anaspec Company choosing this zebrafish photo named "Female Zebrafish-01_worth $200 US dollars" on one Z-Fish Antibodies ad in the 2011 zebrafish meeting brochure (www.union.wisc.edu/zebrafish/).

 

The zebrafish meeting is "4th Strategic Conference of Zebrafish Investigators" to be held January 29th - February 2nd, 2011 at Asilomar Conference Center in Pacific Grove, California.

 

0214.2010 Update

Thanks for the Notre Dame University's NDeRC (Notre Dame extended Research Community) choose this photo as the main photo along their BioEyes website (erc.nd.edu/blogs/bioeyes/) and thier Collaborations website (erc.nd.edu/collaborations/).

 

0422.2009 Update

Thanks for the CBCnews Canada choosing this zebrafish photo named "Female Zebrafish-01_Worth $200 US dollars" on their official website.

The homegape URL is "http://www.cbc.ca/news".

The application article is "The eyes have it" and its URL is "http://www.cbc.ca/news/canada/the-eyes-have-it-1.791619"

 

Acknowledgement :

Thanks for the "Lin Li-Yih Lab"* supplied the zebrafish.

* Lin Li-Yih Lab, The Department of Life Science, The National Taiwan Normal University, ROC.

 

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The following descriptions of zebrafish quote from wikipedia website (URL: en.wikipedia.org/wiki/Zebrafish). All Rights are belonging to "Wikipedia website".

 

Zebrafish:

 

1.Introduce: The zebrafish, Danio rerio, is a tropical freshwater fish belonging to the minnow family (Cyprinidae). It is a popular aquarium fish, frequently sold under the trade name zebra danio, and is an important vertebrate model organism in scientific research.

 

2.Distribution: The zebrafish is native to the streams of the southeastern Himalayan region., including the countries Pakistan, Bangladesh, Nepal, and Myanmar. It arose in the Ganges region in Eastern India. It commonly inhabits streams, canals, ditches, ponds, and slow-moving to stagnant water bodies, including rice fields. Zebrafish have been introduced to parts of the United States, presumably by deliberate release or by escape from fish farms. They have also been sighted in Colombia.

 

3.Description: The fish is named for the five uniform, pigmented, horizontal blue stripes on the side of the body, all of which extend to the end of the caudal fin. Its shape can be described as fusiform and laterally compressed, with its mouth directed upwards. Males are torpedo shaped and have gold stripes between the blue stripes; females have a larger, whitish belly and have silver stripes instead of gold. Adult females will exhibit a small genital papilla in front of the anal fin origin. The zebrafish can grow to 6.4 centimetres (2.5 in), although it is uncommon for them to grow past 4 centimetres in captivity.

The approximate generation time for the Danio is 3–4 months. It has been observed that there must be a male present in order for ovulation and spawning of eggs to occur. Females are able to spawn as often as 2–3 days with hundreds of eggs being laid in each clutch. Upon release from the mother, developmental steps will be made, however without the presence of sperm growth will stop after the first few embryonic cleavages. Fertilized eggs will almost immediately become transparent, which is an important characteristic yielding D. rerio as a convenient research model. Development rapidly progresses, with precursors to all major organs appearing within 36 hours of fertilization. Hatching will take place anywhere from 48–72 hours post-fertilization, depending on the internal conditions of the embryo itself and the external temperature (ideally 28.5 °C). Swimming and feeding behavior are observed to occur approximately 72 hours post-fertilization. The sex of juvenile zebrafish cannot be distinguished except by dissection, and the genetic sex determinants are not clearly understood. The range of life-span for a zebrafish in captivity is around 2–3 years, although in ideal conditions, they may live up to 5 years. The zebrafish is omnivorous, and it primarily eats zooplankton, insects, and phytoplankton. It can eat a variety of foods if its main sources are not readily available.

 

4.Model organism for development and genetics: Zebrafish chromatophores, shown here mediating background adaptation, are studied by scientists D. rerio are a common and useful model organism for studies of vertebrate development and gene function. They may supplement higher vertebrate models, such as rats and mice. Pioneering work of George Streisinger at the University of Oregon established the zebrafish as a model organism; its importance was consolidated by large scale forward genetic screens (commonly referred to as the Tübingen/Boston screens). The scholarly journal Development devoted an issue to research using the fish in celebration of this landmark. An online database of zebrafish genetic, genomic, and developmental information, the Zebrafish Information Network (ZFIN), has been established. D. rerio is one of the few species of fish to have been flown into space.

A Zebrafish Pigment Mutant. The mutant called bleached blond was produced by insertional mutagenesis. The embryos in the picture are four days old. At the top is a wild-type embryo, below is the mutant. The mutant lacks black pigment in the melanocytes because it fails to synthesise melanin properly.

Research with D. rerio has allowed advances in the fields of developmental biology, oncology, toxicology, reproductive studies, teratology, genetics, neurobiology, environmental sciences, stem cell and regenerative medicine, and evolutionary theory. Perhaps its greatest advantages for use in the laboratory as a model system come from its now sequenced genetic code, well understood, easily observable and testable developmental behaviors, and the availability of well-characterized mutants. Zebrafish embryonic development provides advantages over other vertebrate model organisms as well. Although the overall generation time of zebrafish is comparable to that of mice, zebrafish embryos develop rapidly, progressing from eggs to larvae in under three days. The embryos are large, robust, and transparent and develop externally to the mother, characteristics which all facilitate experimental manipulation and observation. Their nearly constant size during early development facilitates simple staining techniques, and drugs may be administered by adding directly to the tank. Unfertilized eggs can be made to divide, and the two-celled embryo fused into a single cell, creating a fully homozygous embryo.

See link for pigmentation mutants of D rerio: www.nature.com/hdy/journal/v97/n3/fig_tab/6800867f5.html#...

A common reverse genetics technique is to reduce gene expression or modify splicing in zebrafish using Morpholino antisense technology. Morpholino oligonucleotides are stable, synthetic macromolecules that contain the same bases as DNA or RNA; by binding to complementary RNA sequences, they reduce the expression of specific genes. The journal Genesis devoted an issue to research using Morpholino oligos, mostly in D. rerio. Morpholino oligonucleotides can be injected into one cell of a zebrafish embryo after the 32-cell stage, producing an organism in which gene expression is reduced in only the cells descended from the injected cell. However, cells in the early embryo (<32 cells) are interpermeable to large molecules, allowing diffusion of Morpholinos between cells. A known problem with gene knockdowns in zebrafish is that, because the genome underwent a duplication after the divergence of ray-finned fishes and lobe-finned fishes, it is not always easy to silence the activity one of the two gene paralogs reliably due to complementation by the other paralog.

Despite the complications of the zebrafish genome a number of commercially available global platforms for analysis of both gene expression by microarrays and promoter regulation using ChIP-on-chip exist.

Zebrafish have the ability to regenerate fins, skin, the heart, and the brain (in larval stages). Zebrafish have also been found to regenerate photoreceptors and retinal neurons following injury. The mechanisms of this regeneration are unknown, but are currently being studied. Researchers frequently cut the dorsal and ventral tail fins and analyze their regrowth to test for mutations. This research is leading the scientific community in the understanding of healing/repair mechanisms in vertebrates.

 

5.Recent developments: In October 2001, researchers from the University of Oklahoma published the complete mitochondrial DNA sequence of D. rerio. The length of the zebrafish mitochondrial genome is 16,596 base pairs. This is within 100 base pairs of other related species of fish, and it is notably only 18 bp longer than the goldfish (Carassius auratus) and 21 bp longer than the carp (Cyprinus carpio). The zebrafish gene order and content is identical to the common vertebrate form of mitochondrial DNA. It contains 13 protein-coding genes and a noncoding control region containing the origin of replication for the heavy strand. In between a grouping of five tRNA genes, a sequence resembling vertebrate origin of light strand replication is found. In comparing the nucleotide sequence to other vertebrates it is difficult to draw any evolutionary conclusions because it is difficult to determine as to whether base pair changes have adaptive significance.

In December 2005, a study of the golden strain identified the gene responsible for the unusual pigmentation of this strain as SLC24A5, a solute carrier that appeared to be required for melanin production, and confirmed its function with a Morpholino knockdown. The orthologous gene was then characterized in humans and a one base pair difference was found to segregate strongly between fair-skinned Europeans and dark-skinned Africans. This study featured on the cover of the academic journal Science and demonstrates the power of zebrafish as a model organism in the relatively new field of comparative genomics.

In January 2007, Chinese researchers at Fudan University raised genetically modified fish that can detect estrogen pollution in lakes and rivers, showing environmental officials what waterways need to be treated for the substance, which is linked to male infertility. Song Houyan and Zhong Tao, professors at Fudan's molecular medicine lab, spent three years cloning estrogen-sensitive genes and injecting them into the fertile eggs of zebrafish. The modified fish turn green if they are placed into water that is polluted by estrogen.

On August 1, 2007, researchers at University College London said they had grown in the laboratory a type of adult stem cell found in the eyes of fish and mammals that develops into neurons in the retina. These cells could be injected in the eye to treat all diseases where the retinal neurons are damaged — nearly every disease of the eye, including macular degeneration, glaucoma, and diabetes-related blindness. Damage to the retina — the part of the eye that sends messages to the brain — is responsible for most cases of sight loss. The researchers studied Müller glial cells in the eyes of humans aged from 18 months to 91 years and were able to develop them into all types of neurons found in the retina. They were also able to grow them easily in the lab, they reported in the journal Stem Cells. The cells were tested in rats with diseased retinas, where they successfully migrated into the retina and took on the characteristics of the surrounding neurons. Now the team is working on the same approach in humans.

In February 2008, researchers at Children's Hospital Boston reported in the journal Cell Stem Cell the development of a new strain of zebrafish, named Casper, with see-through bodies. This allows for detailed visualization of individual blood stem cells and metastasizing (spreading) cancer cells within a living adult organism. Because the function of many genes are shared between fish and humans, this tool is expected to yield insight into human diseases such as leukemia and other cancers.

In April 2009, Researchers at the Institute of Genomics and Integrative Biology, Delhi announced the sequencing of the wild-type strain of Zebrafish, complete with about 1.7 billion genetic alphabets.

 

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Nikon AF-D 60mm F2.8 Macro

 

可以到以下網址察看斑馬魚資料,資料取自維基百科!

 

en.wikipedia.org/wiki/Zebrafish

 

阿鶴已經上傳一套斑馬魚胚胎發育照片,從剛受精到4.5天的仔魚照片都有。,請點擊以下網址:

 

www.flickr.com/photos/chenhowen/sets/72157618669794787/

 

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There is one biochemistry company pay our lab $200 (US dollars) to get the rights to put this photo on their website and their product fliers. 0808.2009

 

有一家美國生技公司花200美金(約台幣6600元)向實驗室買使用權,之後這張斑馬魚照片會放在該公司的網站和產品封面。 0830.2009

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Taken on August 13, 2007