• Metarhizium anisopliae (conidia, spore)
  • Agrobacterium tumefaciens (bacteria cell)
  • Micro fibril structure.. why the bacteria produces it? ;-)
  • Scale: One micrometer
  • I don't know what are these white spots.
  • This bacteria may use non-polar cell division, so they form some kind of "bud"

Scanning Electron Microscopy

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This photo was later published in my paper:
R.T.D. Duarte, C.C. Staats, M.H.P. Fungaro, A. Schrank, M.H. Vainsten, L. Furlaneto-Maia, C.V. Nakamura, W. de Souza, M.C. Furlaneto. 2007. Development of a simple and rapid Agrobacterium tumefaciens-mediated transformation system for the entomopathogenic fungus Metarhizium anisopliae var. acridum. Letters in Applied Microbiology, v. 44, n. 3, pages 248 - 254.
www3.interscience.wiley.com/journal/117997919/abstract?CR...

Photo from a Scanning Electron Microscopy (SEM) of Metarhizium anisopliae and Agrobacterium tumefaciens. The microscope is a JEOL-JSM-6340F Field Emission SEM, from Universidade Federal do Rio de Janeiro, Brazil.

Magnification: 15000 x

I worked with them at my master's degree. My research was about the transference of DNA from the bacterium (A. tumefaciens) to the fungus cell (M. anisopliae). Here is a photo of them, after 9 hours of co-cultivation in the same medium. The bacteria cells (smaller rod-like) form aggregates around the fungi conidia and hypha.

The image clearly shows some kind of "fibril-like" structures between the bacteria and fungi. We know that the bacteria produce fibril proteins that attaches to the PLANT cells, but we need to make a couple of experiments to conclude if it is the same fibrils for the fungi.

Stregoika, feesta, OldUncleMe, and 13 other people added this photo to their favorites.

  1. Stregoika ages ago | reply

    I like these pics a lot :)

    You could add notes about which parts belong to whom ;)

  2. rubensduarte ages ago | reply

    For more info about how a Scanning Electron Microscopy works, visit this link: www.mos.org/sln/SEM

  3. rubensduarte ages ago | reply

    Your wish is granted, Iris! :-)

  4. Stregoika ages ago | reply

    hihi thanks! :D
    Awesome pic!

  5. scott_rtw ages ago | reply

    Great photo!

    Would you care to add it to Natural History Mystery?

    Please read my Welcome! message when you get there.

  6. elite moon [deleted] ages ago | reply

    please consider adding this photo to the Decomposition pool

  7. CdePaz ages ago | reply

    This would be right in :Microbiology Labs Training
    We would like to see this image in our pool. Please, join us.
    Microbiology Labs Training

  8. TatyVera ZEN ......O_o 100 months ago | reply

    Wow great shot I´m working with this fungus :)

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