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Scanning Electron Microscopy | by rubensduarte
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Scanning Electron Microscopy

This photo was later published in my paper:

R.T.D. Duarte, C.C. Staats, M.H.P. Fungaro, A. Schrank, M.H. Vainsten, L. Furlaneto-Maia, C.V. Nakamura, W. de Souza, M.C. Furlaneto. 2007. Development of a simple and rapid Agrobacterium tumefaciens-mediated transformation system for the entomopathogenic fungus Metarhizium anisopliae var. acridum. Letters in Applied Microbiology, v. 44, n. 3, pages 248 - 254.



Photo from a Scanning Electron Microscopy (SEM) of Metarhizium anisopliae and Agrobacterium tumefaciens. The microscope is a JEOL-JSM-6340F Field Emission SEM, from Universidade Federal do Rio de Janeiro, Brazil.


Magnification: 15000 x


I worked with them at my master's degree. My research was about the transference of DNA from the bacterium (A. tumefaciens) to the fungus cell (M. anisopliae). Here is a photo of them, after 9 hours of co-cultivation in the same medium. The bacteria cells (smaller rod-like) form aggregates around the fungi conidia and hypha.


The image clearly shows some kind of "fibril-like" structures between the bacteria and fungi. We know that the bacteria produce fibril proteins that attaches to the PLANT cells, but we need to make a couple of experiments to conclude if it is the same fibrils for the fungi.


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Uploaded on August 16, 2005