As with the other flower pictures I've posted recently, I've used the technique of taking multiple exposures and changing the focus in each exposure. They are then combined in Photoshop CS4 to give more depth of field than the lens is capable of in just one shot. This technique is explained in the March 2009 issue of Popular Photography.
To see more images using this depth of field enhancing technique please see my Maximum Depth of Field Macros set. www.flickr.com/photos/9422878@N08/sets/72157624044378381/
I have other Iris images in my Iris set www.flickr.com/photos/9422878@N08/sets/72157623861858581/