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Dividing human nucleus movie

Confocal microscope images of live human cells imaged for ~4 hours.

 

These cells are a human cancer cell line. This movie shows the fluorescence from cells expressing a fluorescent protein which stains the nucleus (shown as a grayscale image). The cell which starts in the bottom right of the field undergoes mitosis (cell division) about 1/3rd of the way through. There are other cells which wander into the field, but these don't divide over this timescale.

 

This image nicely shows the separation of the two genomes into the two new nuclei.

 

For division, the cell has to have already copied it's chromosomes, and then disassemble the nuclear membrane, then sort it's chromosomes to correctly separate the pairs into two new daughter cells, which then have to reassemble their nuclear membranes and separate their plasma (outer) membranes. All quite a feat really. Its the nuclear separation that is nice and clear in this video.

 

Image capture on a temperature, CO2 and humidity controlled stage of a Zeiss LSM510 using minimal light input to obtain fluorescence from the fluorescent protein.

 

Movie created from a subportion of the whole field and rendered as an rgb avi in ImageJ.

 

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Uploaded on June 2, 2009