Image from page 331 of "Diseases of the dog and their treatment" (1911)
Publisher: Chicago, Ill. : Alexander Eger
Contributing Library: Webster Family Library of Veterinary Medicine
Digitizing Sponsor: Tufts University
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es, etc., in thestomach. Regarding the presence of sugar in the urine of rabid dogs, thatcannot be said to be of special significance, as it is found to be presentin other diseases. The presence of the corpuscles of Negri in a suspectedanimal, on the other hand, must be regarded as a diagnostic symptom ofgreat importance, particularly as it is a means of making a quick diag-nosis. The horn of Ammon is where these corpuscles are found in thelargest quantities, and it requires one to be thoroughly familiar withthese bodies to recognize them quickly and easily. They arefound in particles of the horn of Ammon fixed in Zenkers fluid, andtreated with a 10 per cent, solution of osmic acid, then washed thoroughlyand then laid in absolute alcohol; this method has the disadvantage of tak-ing some time to accomplish it. The method of Bohne seems to be the mostdesirable. Mode of procedure: sections one-half to three-fourths mm.thick are cut from the horn of Ammon and put in 15 c.c. of acetone and
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A SMEAR PREPARATION OF THE HIPPOCAMPUS MAJOR SHOWING NEGRI BODIES,STAINED BY THE METHOD OF VAN GIESEN, ENLARGED looo DIAMETERS. [St-e RaHes]. Pink : Stained tissue.Blue: Cells. Brick: Negri bodies.Red : Blood corpuscles. RABIES 299 left at a tcmporatiiro of 37° until they become hardened^ then the sec-tions are transferred to liquefied paraffine and left there for an hour at atemperature of 60°, then the sections are put in cold water to which asmall quantity of gum aral3ic is added and put in a stone and the paraffinecarried off, then the sections are colored by means of Manns process,which is to put the sections for one-half to four minutes in a coloringsolution consisting of 35 c.c. of 1 per cent, aqueous solution of methyleneblue and 35 c.c. of 1 per cent, aqueous solution of eosin and 100 c.c.of distilled water. The sections are rinsed with water and then putin absolute alcohol for 15 to 20 seconds, to which has been addedsome caustic soda (to 30 c.c. of alcohol add 5 droi>?
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