UCFlow's photostream

An example of doing an antibody titration using a CD4 antibody coupled
to Alexa 488.

Why not stop over at the UCFlow Image Pool on Flickr (www.flickr.com) and check out some of the photos of data, instruments, and other things of interest. Or, better yet, why not send an image of your own to the flickr pool. All we ask is that it be flow cytometry related. If you want to send your image, simply compose an email to whose60which@photos.flickr.com. The Subject line of the email will be the photo's title, and the body of the email will be a description of the photo (make sure to remove your signature so it doesn't become part of the description). Then just attach the image, and send it along. It will be posted to the flickr pool, and randomly displayed on the facility's home page, ucflow.uchicago.edu

An example of looking at cell death by flow cytometry. The plot on the left identifies cells with an intact membrane, that is, excluding cells with a compromised cell membrane. The second plot looks at cells whose membrane has inverted exposing phosphotidylserines to the outside of the cell, allowing for Annexin V to bind as well has having a depolarized mitochondrial membrane which releases the MitoTracker Red dye from the cell. Apoptotic cells therefore are Annexin V + and MitoTracker Low.

An example of a multicolor assay, showing the ability to detect multiple fluorchrome-coupled antibodies for a complex analysis. This tube has FITC, APC, PECy7, PE, and DAPI included

A zoomed in look at the flowcell of a BD FACSCan illuminated by the 488nm argon laser.