Painted on the ceiling above the main staircase in the Palacio de los Duques de San Carlos, a 16th century renaissance palacio in Trujillo, a town in the Spanish region of Extremadura. The building is now a convent for the Order of St Jerome.
The double headed eagle is the symbol of (amongst many other things) the Habsburg dynasty who ruled Spain in the 16th century. It was the Habsburg Holy Roman Emperor Carlos V who gave permission to the owners of the palace (the Vargas-Carvajal family, whose crest of diagonal and wavy lines sits at the centre) to use this emblem to decorate their home.
The image also includes the cross of the Dominicans, each point of which ends in a fleur-de-lis (an attribute of St Dominic).